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Promega goscript™ 5x rt reaction buffer
Goscript™ 5x Rt Reaction Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscript%E2%84%A2+5x+rt+reaction+buffer/goscript+5x+reaction+buffer/pmc11135205-114-50-55
Average 90 stars, based on 1 article reviews
goscript™ 5x rt reaction buffer - by Bioz Stars, 2026-10
90/100 stars

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Reverse Transcription:

Article Title: Simultaneous construction strategy using two types of fluorescent markers for HVT vector vaccine against infectious bursal disease and H9N2 avian influenza virus by NHEJ-CRISPR/Cas9
Article Snippet: In the reverse transcription (RT) reaction, 8 μL of extracted RNA and 2 μL of dimethyl sulfoxide (Tedia, Ohio, USA) were heated at 100°C for 5 min and then placed in an ice bath for 5 min. Then, the following materials were added to this reaction mixture: 8 μL of GoScriptTM 5X RT reaction buffer (Promega, Madison, WI, USA), 10 μL of 2.0 mM dNTP (SolGent, Daejeon, Korea), 4 μL of MgCl 2 (Promega, Charbonnie‘re, France), 1 μL of 20 units Recombinant RNasin® Ribonuclease Inhibitor (Promega, Madison, WI, USA), 1 μL of GoScriptTM reverse transcriptase, 1 μL of 50 pmol random primer, and 4 μL of diethylpyrocarbonate-treated water (DEPC water; Biosesang, Seoul, Korea); a final volume of 39 μL was obtained.

Recombinant:

Article Title: Simultaneous construction strategy using two types of fluorescent markers for HVT vector vaccine against infectious bursal disease and H9N2 avian influenza virus by NHEJ-CRISPR/Cas9
Article Snippet: In the reverse transcription (RT) reaction, 8 μL of extracted RNA and 2 μL of dimethyl sulfoxide (Tedia, Ohio, USA) were heated at 100°C for 5 min and then placed in an ice bath for 5 min. Then, the following materials were added to this reaction mixture: 8 μL of GoScriptTM 5X RT reaction buffer (Promega, Madison, WI, USA), 10 μL of 2.0 mM dNTP (SolGent, Daejeon, Korea), 4 μL of MgCl 2 (Promega, Charbonnie‘re, France), 1 μL of 20 units Recombinant RNasin® Ribonuclease Inhibitor (Promega, Madison, WI, USA), 1 μL of GoScriptTM reverse transcriptase, 1 μL of 50 pmol random primer, and 4 μL of diethylpyrocarbonate-treated water (DEPC water; Biosesang, Seoul, Korea); a final volume of 39 μL was obtained.



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Promega goscript™ 5x rt reaction buffer
Goscript™ 5x Rt Reaction Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscript%E2%84%A2+5x+rt+reaction+buffer/goscript+5x+reaction+buffer/pmc11135205-114-50-55
Average 90 stars, based on 1 article reviews
goscript™ 5x rt reaction buffer - by Bioz Stars, 2026-10
90/100 stars
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